Knowledge-enriched characterization of mobile populations. Morphological information from photographs can increase towards the richness of flow cytometry info. One example is, the determine displays an in any other case conventional apoptosis assay working with Annexin V and PI, adding mobile imaging to characterize cells in Each individual inhabitants to expose morphologically distinct features. These insights could not have been acquired from multiplex staining by itself.

Irrespective of whether you’re a starter or a professional, explore our means to progress your analysis and experience in flow cytometry.

(B) Uncooked 264.seven macrophages will often be annotated inaccurately with regard to particle depend due to their complexity and noncircular borders. The amount of singlets obtainable for Evaluation raises as soon as the person trains and employs a far more exact product.

The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing by using a significant-velocity brightfield digicam for simultaneous higher throughput flow cytometry and superior resolution brightfield imaging.

"An image is not pretty well worth 1000 dots, nevertheless it really helps you to see photographs. It is excellent to find out images that correlate with staining. As an example, AnnexinV-good cells that happen to be morphologically unique than healthy cells, or CD14+ monocytes which have been more substantial plus more textured than CD3+/CD4+/CD14- lymphocytes.

Additionally, it truly is illuminating to view the number of doublets slip by traditional singlet gates, and fantastic to verify the standing of unexpected double-beneficial gatherings in my Evaluation."

Optimizing gating technique. Even strong handbook singlet gating is error-susceptible and continues to be a subjective decision point in almost all flow cytometry assays. Imaging can be employed to verify and adjust gates to include only one cells of desire.

Steady image good quality even at superior throughput Acoustic focusing in addition to a higher-velocity camera combine to graphic these T cells continuously at small or substantial flow rates. Easily adjust emphasis and digital camera settings to satisfy experimental demands.

Early signs of this contamination had initially been dismissed as debris. Backgating to find new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also helps you to use morphological capabilities to find out fascinating subpopulations that will not be evident from flow cytometry information alone.

Possibly most of all, these events consist of cells of Evidently various phenotypes which could lead to incorrect conclusions with regards to double good situations (especially in unusual populations).

Audio waves allow precise mobile alignment, leading to Outstanding data good quality and ~10X more rapidly sample flow premiums.

Application Be aware: Automated more info image Examination lowers consumer-to-person variability in flow cytometry gating approaches

Leverage the power of Superior technological know-how and innovation to speed up your discoveries. Have confidence in while in the Attune Flow Cytometer loved ones to deliver the reliability, speed, and precision that set your do the job apart.

Adaptable payment plan from Agilent Economical Answers let you receive the significant instrumentation you require for fast task, or to mature your lab.

At the heart of each Attune Flow Cytometer lies our innovative acoustic concentrating technological innovation, which utilizes seem waves to meticulously align cells throughout the flow cell. This modern approach surpasses classic hydrodynamic methods, leading to speedier processing speeds, exceptional facts top quality, and greater sample throughput.

The Attune Xenith cytometer is really a higher-parameter spectral analyzer, furnishing both spectral unmixing and standard flow abilities for complete and flexible mobile Evaluation.

The Attune CytPix Flow Cytometer lets buyers to visualize samples for the duration of acquisition, verify the precision of gating methods, and assist benefits with trustworthy documentation for publication needs.

What seemed to be a homogeneous inhabitants is actually more varied—an insight that needs to be thought of when interpreting success.